Deletion of GBG1/AYR1 Alters Cell Wall Biogenesis in Saccharomyces cerevisiae
Mycobiology
;
: 102-107, 2010.
Article
in English
| WPRIM
| ID: wpr-729538
ABSTRACT
We identified a gene for beta-1,3-glucan synthesis (GBG1), a nonessential gene whose disruption alters cell wall synthesis enzyme activities and cell wall composition. This gene was cloned by functional complementation of defects in beta-1,3-glucan synthase activity of the the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. Disruption of the GBG1 gene did not affect cell viability or growth rate, but did cause alterations in cell wall synthesis enzyme activities reduction of beta-1,3-glucan synthase and chitin synthase III activities as well as increased chitin synthase I and II activities. GBG1 disruption also showed altered cell wall composition as well as susceptibility toward cell wall inhibitors such as Zymolyase, Calcofluor white, and Nikkomycin Z. These results indicate that GBG1 plays a role in cell wall biogenesis in S. cerevisiae
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Saccharomyces
/
Saccharomyces cerevisiae
/
Benzenesulfonates
/
Organelle Biogenesis
/
Complement System Proteins
/
Cell Survival
/
Cell Wall
/
Chitin Synthase
/
Genes, vif
/
Clone Cells
Limits:
Humans
Language:
English
Journal:
Mycobiology
Year:
2010
Type:
Article
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