Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis
Mycobiology
;
: 266-271, 2015.
Article
in English
| WPRIM
| ID: wpr-729637
ABSTRACT
Several types of yeasts were isolated from wild flowers around Jangseong Lake in Jeollanam-do, Republic of Korea and identified by comparing the nucleotide sequences of the PCR amplicons for the D1/D2 variable domain of the 26S ribosomal DNA using Basic Local Alignment Search Tool (BLAST) analysis. In total, 60 strains from 18 species were isolated, and Pseudozyma spp. (27 strains), which included Pseudozyma rugulosa (7 strains) and Pseudozyma aphidis (6 strains), was dominant species. Among the 60 strains, Bullera coprosmaensis JS00600 represented a newly recorded yeast strain in Korea, and its microbiological characteristics were investigated. The yeast cell has an oval-shaped morphology measuring 1.4 x 1.7 microm in size. Bullera coprosmaensis JS00600 is an asporous yeast that exhibits no pseudomycelium formation. It grew well in vitamin-free medium as well as in yeast extract-malt extract broth and yeast extract-peptone-dextrose (YPD) broth, and it is halotolerant growing in 10% NaCl-containing YPD broth.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Yeasts
/
DNA, Ribosomal
/
Base Sequence
/
Lakes
/
Polymerase Chain Reaction
/
Flowers
/
Republic of Korea
/
Korea
Type of study:
Diagnostic study
/
Prognostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Mycobiology
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS