Analysis of Cultural Characteristics and Phylogenic Relationships of Collected Strains of Pholiota species

ABSTRACT

Cultural characteristics and phylogenic relationships were investigated and classified among collected strains in Pholiota spp. which contain P. adiposa, P. squarrosa, P. nameko etc. They were tested on the four different media (PDA, MCM, YM, MEA) and sawdust (Alder, Oak, Pine, Popular) substrates. There was a little variation according to the media and sawdust substrates, although PDA and popular sawdust substrate seemed to be better. Most strains showed white colonies, but some strains were brown. Mycelial growth length differed according to the strains. To classify species, the internal transcribed spacer regions (ITS) of the ribosomal DNA (rDNA) repeats from Pholiota spp. were amplified using polymerase chain reaction (PCR) and then sequenced. According to the analysis of ITS sequences, they were classified into five clusters. Their spacer regions were 644~700 nucleotides in length. The reciprocal homologies of each ITS region among these strains were ranged from 49.6~99.9%. The phylogenic analysis might give a criterion to classify species in the collected strains.