Isolation and Characterization of Cryptococcus neoformans from Environmental Sources in Busan
Mycobiology
;
: 188-193, 2005.
Article
in English
| WPRIM
| ID: wpr-729822
ABSTRACT
Twenty nine samples of pigeon droppings (n = 12) and soil contaminated with avian excreta (n = 19), collected from different sites in Busan, were examined for isolation and characterization of Cryptococcus neoformans. Of these samples, 5 strains of C. neoformans were recovered from pigeon droppings (5/12 41.7%). All isolates were belonged to C. neoformans var. grubii (serotype A). The extracellular enzyme activities of the strains by using the API-ZYM system showed two different enzymatic patterns. The genetic variability among C. neoformans isolates was analyzed by random amplified polymorphic DNA (RAPD) using three 10-mer primers. Two different RAPD patterns, which clearly distinguished the isolates, were identified. Analysis of RAPD patterns provided a good characterization of environmental strains of C. neoformans serotype A as a heterogeneous group and were in good agreement with enzymatic profiles.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Columbidae
/
Soil
/
DNA
/
Cryptococcus
/
Cryptococcus neoformans
Language:
English
Journal:
Mycobiology
Year:
2005
Type:
Article
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