An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
Mycobiology
;
: 434-437, 2017.
Article
in English
| WPRIM
| ID: wpr-730007
ABSTRACT
An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus—Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 µg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parents
/
Polysaccharides
/
RNA
/
Polymerase Chain Reaction
/
Mycelium
/
Agaricales
/
Real-Time Polymerase Chain Reaction
/
Fungi
/
Methods
Limits:
Humans
Language:
English
Journal:
Mycobiology
Year:
2017
Type:
Article
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