Development of a Rapid Molecular Detection Marker for Colletotrichum species with AFLP
Mycobiology
;
: 123-127, 2004.
Article
in English
| WPRIM
| ID: wpr-730040
ABSTRACT
Sweet persimmons have been increasingly cultivated in the southern part of Korea. However, anthracnose disease caused by Colletotrichum species is one of the major hindrances in cultivation and productions. In this study, we used polymerase chain reaction(PCR) to detect Colletotrichum species with the AFLP(amplified fragment length polymorphism) method. In AFLP, we used E3(5'-GACTGCGTACCAATTCTA-3') and M1(5'-GATGAGTCCTGAGTAACAG-3') primer combination and, as a result, 262 bp segment was observed in Colletotrichum species only. Specific PCR primers were designed from the sequence data and used to detect the presence of the fungus in genomic DNA isolated from symptomless sweet persimmon plants. Based on sequence data for specific segments, Co.B1(5'-GAGAGAGTAGAATTGCGCTG-3') and Co.B2(5'-CTACCATTCTTCTA GGTGGG-3') were designed to detect Colletotrichum species. The 220 bp segment was observed in Colletotrichum species only, but not in other fungal and bacterial isolates.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Polymerase Chain Reaction
/
Colletotrichum
/
Diospyros
/
Fungi
/
Korea
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Mycobiology
Year:
2004
Type:
Article
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