Correlation of serum miR-146a with nuclear factor -κB and vascular endothelial growth factor in diabetic retinopathy patients / 国际眼科杂志(Guoji Yanke Zazhi)

ABSTRACT

@#AIM:To investigate the correlation of serum miR-146a with nuclear factor-κB(NF-κB)and vascular endothelial growth factor(VEGF)in diabetic retinopathy patients. <p>METHODS: A total of 100 patients with T2DM treated in our hospital from July 2016 to December 2017 were assigned into T2DM patients with DR(DR group, <i>n</i>=32)and T2DM patients without DR(T2DM group, <i>n</i>=68). Thirty healthy volunteers were selected as control group. Real-time PCR was used to examine the expression of miR-146a. Enzyme linked immunosorbent assay was used to detect the levels of NF-κB and VEGF. The correlation between miR-146a and NF-κB and VEGF was analyzed. <p>RESULTS: Compared with the control group, HbA1c in T2DM group and DR group increased(<i>t</i>=6.822, 5.709; <i>P</i><0.001), FBG increased(<i>t</i>=8.889, 7.923; <i>P</i><0.001), 2hPBG increased(<i>t</i>=6.646, 5.514; <i>P</i><0.001). Compared with T2DM group, the duration of diabetes in DR group was longer(<i>t</i>=2.431, <i>P</i>=0.017). Compared with the control group, serum miR-146a in T2DM and group DR significantly decreased(<i>t</i>=3.967, 7.169; <i>P</i><0.001), and the DR group was lower than that in the T2DM group(<i>t</i>=4.444, <i>P</i><0.001). Compared with the control group, the serum NF-κB in the T2DM and DR group increased significantly(<i>t</i>=6.063, 14.851; <i>P</i><0.001), VEGF increased significantly(<i>t</i>=7.613, 12.943; <i>P</i><0.001), NF-κB and VEGF in DR group were larger than those in T2DM group(<i>t</i>=11.406, 7.560; <i>P</i><0.001). Pearson analysis showed that miR-146a was negatively correlated with NF-κB and VEGF(<i>r</i>=-0.503, -0.574; <i>P</i><0.05). <p>CONCLUSION: The serum miR-146a in DR patients significantly decreased, the NF-κB and VEGF significantly increased. MiR-146a may be involved in the pathogenesis of DR by mediating inflammatory reaction and vascular proliferation.