Autophagy of Human Tenon's Capsule Fibroblasts Induced by Mitomycin-C
Journal of the Korean Ophthalmological Society
;
: 1337-1343, 2011.
Article
in Korean
| WPRIM
| ID: wpr-73140
ABSTRACT
PURPOSE:
The present study investigated whether an autophagic process is involved in the apoptotic death of human tenon's capsule fibroblasts (HTCFs) caused by mitomycin-C.METHODS:
An autophagic phenotype was tested using fluorescence microscopy and flow cytometry with specific biological staining dyes including monodansylcadaverine and acridine orange and microtubule-associated protein 1 light chain 3 (LC3).RESULTS:
Treatment with mitomycin-C (0.4 mg/ml) increased the acidic vesicular organelles of tenon's capsule fibroblasts in a time dependent manner. Mitomycin-C induced both LC3-II cleavage and beclin-1 expression. 3-MA, a pharmacological inhibitor of autophagy, inhibited the mitomycin-C induced increase of acidic vesicular organelleS.CONCLUSIONS:
Autophagy was induced with 0.4 mg/ml mitomycin-C in tenon's capsule fibroblasts. And, autophagic mechanisms may be involved in the early stage of apoptosis of fibroblasts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Autophagy
/
Acridine Orange
/
Organelles
/
Cadaverine
/
Mitomycin
/
Apoptosis
/
Coloring Agents
/
Tenon Capsule
/
Fibroblasts
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS