Homoharringtonine Prompt Apoptotic Induction of Imatinib in CML Cells / 沈阳医学院学报
Journal of Shenyang Medical College
;
(6): 332-335, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-731807
ABSTRACT
Objective:
To investigate the apoptotic induction effects of homoharringtonine (HHT) combined with imatinib (STI571) on human chronic myeloid leukemia (CML) KU812 cells.Methods:
KU812 cells were treated with HHT or/and STI571. Total cell numbers were counted by using hemocytometer. Apoptotic cells were determined by using fluorescence microscope and FACS after staining with AO-EB and PI respectively. The cleavage of poly ADP-ribose polymerase (PARP) and the expression of anti-apoptotic proteins were determined by Western blot.Results:
HHT could prompt the apoptotic induction of imatinib in KU812 cells, which was in a time and dose dependent manner. Apoptosis induced by HHT and imatinib was correlated with the down-regulation of Bcl-XL and the cleavage of PARP.Conclusion:
HHT prompt apoptotic induction of imatinb in KU812 cells and the combination of HHT with imatinib will provide a more effective strategy for the clinical treatment of CML.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Shenyang Medical College
Year:
2016
Type:
Article
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