SOME ISSUE TREATING LUNG DISORDER ON EXPERIMENTAL ANIMAL MODEL BY“SOROOL-4” TRADITIONAL DRUG / Монголын эм зүй, эм судлал

ABSTRACT

Background: In Mongolia, leading cause of morbidity prevalence is respiratory system disease by 2010. The prevalence is 1027,73 per 10000 people. Comparison to 2000, it has been increased by 1,5-2 times. Another word, it shown that respiratory diseases hadn’t been decreased, but progressively increased in the last 10 year.By WHO estimation in 2008, future trend of mortality rate of the respiratory system disease would taken 3rd place in the leading causes of the mortality by 2030. Most common symptoms of the respiratory diseases are cough and sputum.Aim of the study Aim of this study is to investigate some effects Sorool-4 drug on some respiratory diseases.Method of study Experimental research method was used in this study. 30 laboratory white mouse, each weigh is 18-22 grams and 40 Vistar species rats, each weigh is 180-220 g were used in the study in where Inner Mongolian National University of China.0.25% Ammonium hydrochloridium solvent was inhaled to each mices for 10 seconds. After 3 day inhalation procedure, Sorool-4 drug was injected 10 grams per kg to mices, and Libexin was administered 4.3mg/kg per one time in next 3 day. During these 3 days, we measured cough for 3 minutes to each mices by ZOLY- 3 devices in everyday.White mices were classified into 3 groups, and then0.1 ml/10gram phenol aldehyde solution 0.2-0.25 ml per each mice injected to abdominal cavity. After 3 day injection, sorool-4 was administered 10 gram per kg and bromhexine 0.12 mg per kg, 3 times a day, for 3 days.Bacterial colony Coagulase positive S.aureus DU 5789 and coagulase negative mutant DU5843 species were used in this study. These bacterias has stored in-700C in brain and heart infusion, and 10% glycerol and 5% non-fat milk were feed until used.Culture According to CASH method, some changes were made. S.aureus was cultured in the TSA, BBL agar in +370C for 24 hours and diluted with pure non- endotoxin saline and centrifuged at 3000rpm for 7 days, +40C, 10 min per day. And then again diluted with cold pure saline, and diluted to 2х109-4х109 CFU ml by turbid meter. Prepared suspension has warmed to 450C, and mixed 10 ml suspension with 4% 10ml melted NOBLE agar.Experimental model 0.2-10ml/10ml/g agar wasinjected to every mice tail vein.To develop cough model, 0.25% ammonium hydrochloridium solution was inhaled 10 sec for 3 days in YLS-8A device. After 3 day, in three groups, mices of the each group coughed mean 25.6±3.6 amount. Sorool-4 drug and Libexin were administered 10mg/kg to treatment group 1 and 4.3 mg/kg to treatment group 2, each respectively. After administration these drugs, counted cough amount of each mice for 3 min in YLS- 8A device in 3 day.Conclusion: Because of amount of sputum of treatment group 1 and 2 is more than control group, Sorool-4 is not efficient as same as bromhexine, but this showed that it has mucus expectorant effect.As shown results, sorool-4 has anti-inflammatory effect. It would be more effective using sorool-4 with antibiotic.