Inhibitory effect of phospholipid complex nanocapsules modified triptolide on glioma cells / 国际生物医学工程杂志

ABSTRACT

Objective To investigate the anti-glioma treatment effect of triptolide modified by phospholipid complex nanocapsule encapsulation technology in vitro.Methods Human glioma U87 ceils were used to investigate the uptake efficiency of tumor cells to nano-microcapsules TPL and TPL.MTT method was used to detect the proliferation inhibition rate of U87 cells by nano-microcapsule TPL.The levels of ATP and ROS in U87 cells were measured to reflect cell proliferation and apoptosis effect of nano-microcapsules TPL.Results Glioma cells are sensitive to TPL and nano-microcapsule TPL.Compared with single TPL,nano-microcapsule TPL has better glioma cell inhibitory effect and anti-tumor activity (P＜0.05),and has significantly reduced ATP level (P＜0.05) and increased ROS level (P＜0.05) in U87 cells.Conclusions After being encapsulated by nanocapsule technology,TPL has good glioma cell targeting and can significantly enhance the inhibitory effect of drug-loaded liposomes on glioma cell proliferation.