Establishment of an acute graft-versus-host disease animal model in EL4 T-cell leukemia/lymphoma mice / 中华实用儿科临床杂志

ABSTRACT

Objective To establish an acute graft-versus-host disease (aGVHD) model in EL4 T-cell leukemia/lymphoma mice,for providing experimental model to prevent aGVHD while maintaining graft-versus-leu1 mia (GVL) effect during allogeneic bone marrow transplantation (Allo-BMT).Methods Male BALB/c (H-2Kd)mice were used as donors and female C57BL/6(H-2Kb) mice were used as recipients.C57BL/6 hosts were given 9.5 Gy lethal total body irradiation at 4 hours prior to transplantation.Mice were randomly assigned into 3 groups and each group contained 17 recipientsThe recipients in radiation group were injected with 0.2 mL RPMI 1640 as control.The recipients were injected with donor bone marrow cells (5 × 106/mouse) and splenocyte for aGVHD(5 × 106/mouse).The recipients in EL4 T-cell leukemia/lymphoma aGVHD group were injected with donor bone marrow cells (5 × 106/mouse) and plus 500 EL4 cells (5 × 106/mouse).General state,life span and histopathology of the recipient mice and detected chimera were observed.Results The results showed that the mean survival time in radiation group was (10.10 ± 0.43) days,in aGVHD group was(28.12 ± 5.01)days,in EL4 T-cell leukemia/lymphoma aGVHD group was (31.05 ±5.48) days.The mean survival time in aGVHD group and EL4 T-cell leukemia/lymphoma aGVHD group were significantly longer than that in radiation group(all P ＜ 0.01),but there was no significant difference between aGVHD group and EL4 T-cell leukemia/lymphoma aGVHD group (P ＞ 0.05).Histopathological analysis in several target organs (skin,liver and small intestine) confirmed the presence of sever GVHD in aGVHD group and EL4 T-cell leukemia/ lymphoma aGVHD group.Pathological examination showed disorganization of normal tissues and leukemic cell infiltration in EL4 T-cell leukemia/lymphoma aGVHD group.Conclusion The EL4 T-cell leukemia/lymphoma aGVHD mice model is reliable to the experimental research of aGVHD.