Seroprevalence of Cytomegalovirus infection in children aged 0-8 years in Nanjing / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the seroprevalence of Cytomegalovirus (CMV) infection and ages of the children in primary for better understanding the status of CMV infection in China with evidence-based medicine.Methods Total 837 children randomly selected from Nanjing Children's Hospital,from Jun.to Sep.in 2011,including 513 boys and 324 girls,aged from 1 day to 8 years,with mean age of 3.6 years old,were recruited.Serum samples were tested for CMV IgM,CMV IgG,and CMV IgG avidity index using commercial enzyme-linked immunosorbent assay kits.Results Of the total serum samples from 837 children,690 cases were CMV IgG positive.The overall seroprevalence of CMV IgG was 82.4％,with 83.2％ (427/513 cases) and 81.2％ (263/324 cases) in boys and girls,respectively.There was no significant difference in the seroprevalence of CMV IgG between boys and girls (x2 =0.584,P =0.445).Of the 92 infants less than 6 months old,86 cases were CMV IgG positive,and the positive rate was 93.5％.The prevalence gradually declined in infants after 7 months old,fell to the lowest level of 66.7％ at age of 9 months,and then constantly maintained around 80.0％ from 1 to 8 years old (x2 =15.4,P ＜ 0.001).CMV IgM in 352 serum samples were tested,and 23 (6.5％) cases were CMV IgM positive.The positive rate peaked in infants at age of 2-3 months (7/12 cases,58.3％),then decreased over the age and none of the children older than 6 years were IgM positive (x2 =5.1,P ＜0.001).Furthermore,the CMV IgG avidity index assay was performed in all 23 cases of IgM positive individuals to estimate the primary CMV infection rate.The results showed low avidity index (＜ 30％) in 13 infants,the primary infection rate was 56.5％ (13/23 cases).Most subjects with primary infection (n =7,53.8％) were younger than 1 year old.Conclusions The current seroprevalence of CMV IgG in children in China was around 80.0％,lower than that in adults,and the primary CMV infection mostly occurred before 3 months of age.