Experimental study of bone marrow mesenchymal stem cell transplantation for cerebral ischemic reperfusion injuries / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the therapeutic effect of bone marrow mesenchymal stem cell (MSC)transplantation on cerebral ischemic reperfusion injury.Methods Sprague-Dawleye rat models of cerebral ischemiareperfusion injury were established with suture method.Ninety-six rats were divided into 4 groupsmodel group,MSC transplantation group,control group (injection of phosphate buffer saline,PBS) and sham operation group,with 24 rats in each group.The 6th generation of bone marrow MSCs cultured in vitro were selected and labeled with hoechst 33342,and then transplanted into rats through Penile vein 24 hours after the establishment of model.Equivalent PBS was injected into the control group.Neurological severity score (NSS) was assessed on the 2nd day,3rd day,4th day,8th day,15 th day,and the 22th day in each group.The plantation of MSC in the brain tissue was observed by fluorescence microscope.Furthermore,serial paraffin sections were subsequently stained with 2,3,5-triphenyl tetrazolium chloride,and immunohistochemistry stain with Nestin,neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP),which could be used as the specific markers of neural stem cells,neurons and astrocytes.Results The distribution of blue fluorescent in the impaired brain tissue could be seen at 2 days after transplantation of MSC,and it reached the peak at the 15th day.The result of immunohistochemistry showed that the cells positive for Nestin,NSE or GFAP in the impaired local brain tissue were increased greatly in the MSC transplantation group compared with the model group and control group,and the differences were statistically significant (all P ＜ 0.05).The scope of cerebral infarction in the impaired brain tissue diminished from the 8th day in the MSC transplantation group,the score of NSS was also decreased evidently,the differences were statistically significant compared with the model group and control group (all P ＜ 0.05).Conclusions Bone marrow MSC transplantation can increase the number of neural stem cells,neurons and astrocytes in the location of cerebral ischemic reperfusion injuries,which may promote the repairment of nerve injury.