Influence of different T cell activators on the expression of Foxp3 in bronchial asthma / 中华实用儿科临床杂志

ABSTRACT

Objective To explore the influence of different T cell activators on the expression of Foxp3 in bronchial asthma.Met-hods Peripheral blood monouclear(PBMCs) were isolated from the 30 children suffering from mild persistent asthma allergic to house dust mite (HDM).PBMCs of those children were divided into 2 groups.One group was stimulated with HDM extracts (HDM group) and the other was stimulated with phorbol-12-myristate-13-acetate (PMA) extracts (PMA group).After 48 hours of in vitro stimulation with HDM extracts or PMA extracts,the levels of CD4 + CD25 + T cells,CD4 + CD25 + Foxp3 + T cells and CD4 + CD25 + Foxp3 + IL-10 + T cells were measured by flow cytometry.Results The levels of CD4 + CD25 + T cells significantly decreased in the HDM group[(4.59 ± 1.10) ％] compared with those in the PMA group[(41.24 ±7.95)％] (P ＜0.0001).The levels of CD4+ CD25 + Foxp3 + T cells significantly decreased in the HDM group [(18.08 ± 1.82) ％] compared with those in the PMA group [(90.26 ± 3.63) ％] (P ＜ 0.0001).The levels of CD4 + CD25 + Foxp3 + IL-10 + T cells had no statistical significance in the HDM group [(37.62 ± 3.23) ％] compared with those in the PMA group [(38.33-± 3.95) ％] (P ＞ 0.05).Conclusion Nonspecific T cell activators can increase the expression of Foxp3 in bronchial asthma.