Frequency of Extended-spectrum beta-lactamase (ESBL) and AmpC beta-lactamase Genes in Escherichia coli and Klebsiella pneumoniae over a Three-year Period in a University Hospital in Korea / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 616-623, 2010.
Article
in Korean
| WPRIM
| ID: wpr-73308
ABSTRACT
BACKGROUND:
The aim of this study was to determine the yearly prevalence and genotype distribution of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae collected over a 3-yr period in Gwangju, Korea.METHODS:
Clinical isolates of E. coli and K. pneumoniae collected at Chosun University Hospital from September 15, 2005 to September 14, 2008 were evaluated. Antimicrobial susceptibility testing was performed using the Vitek II system (bioMerieux, USA) and agar dilution methods. Screening for ESBL and AmpC beta-lactamase genes was performed using PCR amplification of plasmid DNA followed by direct sequencing of the PCR products.RESULTS:
The percentage of ESBL-producing isolates was 12.6% (196/1,550) for E. coli and 26.2% (294/1,121) for K. pneumoniae. The ESBL gene sequencing results showed that the most prevalent ESBL types were CTX-M (93.5%) and SHV (12.9%) in E. coli, and SHV (73.2%) and CTX-M (46.3%) in K. pneumoniae. The most common ESBL in E. coli was CTX-M-15-like, followed by CTX-M-14-like, SHV-2a-like, and SHV-12-like. The most prevalent ESBL type in K. pneumoniae was SHV-12, followed by CTX-M-14-like and CTX-M-15-like. Fifty-one percent (21/41) of ESBL-producing K. pneumoniae with ESBL types verified by sequencing also had DHA-1-like AmpC beta-lactamases. However, none of the ESBL-producing E. coli was positive in the AmpC beta-lactamase PCR analysis.CONCLUSIONS:
In this study, the most common types of class A ESBLs identified were CTX-M-15-like in E. coli and SHV-12-like in K. pneumoniae.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacterial Proteins
/
Beta-Lactamases
/
Microbial Sensitivity Tests
/
Sequence Analysis, DNA
/
Drug Resistance, Bacterial
/
Escherichia coli
/
Republic of Korea
/
Gene Frequency
/
Genotype
/
Hospitals, University
Limits:
Humans
Country/Region as subject:
Asia
Language:
Korean
Journal:
The Korean Journal of Laboratory Medicine
Year:
2010
Type:
Article
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