Influence and mechanism of mitochondrial permeability transition pore and cytochrome C in process of HL-60 cell apoptosis induced by desferrioxamine / 中华实用儿科临床杂志

ABSTRACT

Objective To explore the influence of mitochondrial permeability transition pore opening and cytochrome C (Cyt C) being discharged on the apoptotic mechanism of HL-60 cell induced by desferrioxamine (DFO),so as to provide scientific basis for the clinicians to adopt the strategy of iron deprivation to treat human leukemia.Methods HL-60 cells were co-cultivated with various concentration of DFO for 24-72 hours,then the apoptotic cells and the changes of mitochondrial membrane potential(△Ψm) were examined by means of flow cytometry(FCM),and Cyt C in cytoplasm was detected by way of celluar immunohistochemistry.Results The cell apoptotic rate assumed rising tendency as the highest rate could be up to (44.10 ± 6.3 1) ％,and the effect was of time-and-dose dependence (P ＜0.01).FCM could detect the △Ψm declining(P ＜ 0.05),and the Cyt C positive cell rate was higher than the controls,the differences were of statistical significance(P ＜ 0.05).Conclusions DFO can induce the HL-60 cells apoptosis,and the possible mechanism is that DFO make the mitochondrial permeability transition pore open and get Cyt C discharged from the mitochondria.