Effect of γ-Vitamin E on interleukin-4 and interleukin-13 levels in serum and bronchoalveolar lavage fluid in mice with asthma / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the effect of γ-Vitamin E (γ-VitE) on levels of IL-4,IL-13 in serum and bronchoalveolar lavage fluid in mouse with asthma.Methods Thirty healthy Balb/c mice were randomly divided into 3groups(10 mice in each group)asthma group,γ-VitE group and control group.In asthma group and γ-VitE group,20mice were sensitized by exposured to aerosolized ovalbumin and challenged with the same antigen to establish asthma model.Rats in asthma group were not treated with γ-VitE,rats in γ-VitE group were treated with γ-VitE.Rats in control group were treated with normal saline instead of aerosolized ovalbumin.The total cell,acidophilic leukocytes,neutrophils,macrophages and lymphocyte were observed and counted by microscopic after Wright-Giemsa stain.IL-13,IL-4and IgE in serum and bronchoalveolar lavage fluid were measured by ELISA assay.Results Mice sensitized by aerosolized ovalbumin appeared nose spasm itch,sneezing and catching the ear,the symptom were alleviated after γ-VitE intervention.The cell numbers of γ-VitE group were less than those in asthma group,the differences were significant (all P ＜0.05).The ELISA test result showed that the expression of IL-4 and IL-13 in asthma group and γ-VE group were significantly higher than those in control group (all P ＜ 0.05).The levels of IL-4,IL-13 and IgE in γ-VitE group were significantly lower than those in asthma group (all P ＜ 0.05).Conclusions γ-VitE can inhibit the levels of IL-4 and IL-13 in asthma mouse,and reduce the level of IgE.γ-VitE has a certain effect in treatment of asthma.