Role of inflammation and free radical in nerve cell apoptosis in newborn rats with hypoxic-ischemic brain damage / 中华实用儿科临床杂志

ABSTRACT

Objective To explore the changes of tumor necrosis factor-α (TNF-α),nuclear factor kappa B (NF-κB),free radical in brain tissue in newborn rats with hypoxic-ischemic brain damage(HIBD),to clarify the role of TNF-α,NF-κB,free radical in HIBD.Methods The 56 SD neonatal rats were randomly divided into control group and hypoxic-ischemic 6 h,12 h,24 h,48 h,72 h,7 d groups.The conventional method was used to establish HIBD model.The level of TNF-α was measured by enzyme-linked immumosorbent assay,NF-κB was detected by electrophoretic mobility shift assay.The neuron apoptosis was identified by flow cytometry.Superoxide dismutase(SOD),molondialdehyde (MDA),glutathione peroxidase(GSH-Px) were identified by spectrophotography.Results The rate of neuron apoptosis,the contents of TNF-α,NF-κB,MDA in brain tissue were significantly higher in 6 h,12 h,24 h,48 h,72 h groups than those in contral group(all P ＜0.05),and the apoptosis,MDA reached the peak at the 24 h after hypoxia,TNF-α reached the peak at the 12 h after hypoxia,NF-κB reached the peak at the 48 h after hypoxia.But the content of SOD and the activity of GSH-Px in hypoxic-ischemic group were significantly lower than those in control group at 6 h,12 h,24 h,48 h,72 h after hypoxia.And the rate of neuronal apoptosis was positively correlated with TNF-α,NF-κB and MAD (all P ＜ 0.05),negatively correlated with GSH-Px and SOD (all P ＜ 0.05),the contents of TNF-α,NF-κB were positively correlated with MAD (all P ＜ 0.05),negatively correlated with the content of SOD and the activity of GSH-Px (all P ＜ 0.05).Conclusions TNF-α,NF-κB,free radical are early changed after HIBD,So they play an important role in HIBD,inflammation and free radical can promote brain damage.