Effects of matrine on apoptosis and nuclear factor kappaBp65 activity of HL-60 cells induced by pirarubicin / 中华实用儿科临床杂志

ABSTRACT

Objective To explore the effects of matrine on apoptosis and nuclear factor(NF)-κBp65 activity of HL-60 cells induced by pirarubicin (THP).Methods Effects of the apoptosisthe HL-60 cells in blank control group were cultured 14 hours with RPMI 1640; groups of different concentration drugsmatrine0.1 μmol/L,1.0 μmol/L,10.0 μmoL/L and THP1.0 μmol/L,10.0 μmol/L,100.0 μmol/L.The apoptosis of HL-60 cells were tested by fluorescence double colour painting and flow cytometry (FCM).The NF-κBp65 activity of HL-60 cells were determined by FCM.Results The rates of apoptosis of HL-60 cells were (7.14 ± 2.95) ％,(12.34 ± 2.55) ％,(19.3 ±2.31)％ and (31.78 ±4.31)％,(47.25 ±5.27)％,(56.49 ±1.59)％ in matrine 0.1 μmol/L,1.0 μmol/L,10.0 μmol/L and THP 1.0 μmoL/L,10.0 μmol/L,100.0 μmol/L groups,compared to blank control group (6.46 ± 1.45) ％,there were significant difference (F =257.72,677.19 ; all P ＜ 0.001) ; (70.17 ± 5.68) ％ in matrine 0.1 μ mol/L +THP 1.0 μ moL/L group[vs the THP 1.0 μmoL/L group (31.78 ±4.31)％,(t =38.94,P＜0.001)].The rates of NF-κBp65 activity of HL-60 cells in matrine 0.1 μmol/L,1.0 μmol/L,10.0 μmol/L and THP 1.0 μmol/L,10.0 μmol/L,100.0 μmol/L groups were(8.34 ± 1.52)％,(7.11 ± 1.29)％,(4.78 ±0.31)％ and (16.21 ± 1.20) ％,(23.98 ± 3.21) ％,(32.44 ± 2.89) ％,with the control group (8.44 ± 2.20) ％,there were significant difference(F =65.35,P ＜ 0.001 ; F =674.11,P ＜ 0.001) ; (12.01 ± 2.27) ％ in matrine 0.1 μmol/L + THP 1.0 μmol/L group [with (16.21 ± 1.20) ％ of THP 1.0 μmol/L group(t =21.42,P ＜ 0.001)].Conclusions The apoptosis of HL-60 cell is induced by matrine and pirarubicin in a dose-dependent manner.NF-κBp65 activity of HL-60 cell is inhibited by matrine and increased by pirarubicin in a dose-dependent manner.Matrine can enhannce the effect of induction by pirarubicin of apoptosis of HL-60 cell,and decrease activity of NF-κBp65 by pirarubicin.