The role and mechanism of TRAP1 in the development of esophageal cancer / 实用肿瘤学杂志

ABSTRACT

Objective The aim of this study was to investigate the role and mechanism of tumor necrosis factor receptor-re-lated protein 1(TRAP1)in the progression of human esophageal cancer. Methods Immunohistochemistry was used to detect the ex-pression of TRAP1 and S100A8 in human esophageal cancer tissues. A stably knocked-down TRAP1 cell line was established in the esophageal cancer KYSE150 cell line,the proliferation ability was detected by CCK-8,the transfer ability was detected by Transwell, and apoptosis was detected by flow cytometry. We conducted a gene profiling study to detect the expression of genes related to tumor progression. The expression of TRAP1 downstream genes-E-Cadherin,N-Cadherin and S100A8 was detected by Real-Time fluo-rescent quantitative PCR. Results The expression of TRAP1 in esophageal carcinoma was significantly higher than that in adjacent tissues and correlated with S100A8(χ2=4. 141,P<0. 001). The KYSE150 cell line with down-regulated of TRAP1(KYSE150-TRAP1)was established,and the expression of TRAP1 was down-regulated by 85% ,cell invaded ability was decreased by 46% ,no changes of cell proliferation and apoptosis were observed,when compared to the KYSE150 control cells. The expression level of E-cadherin was increased by 19% ,and the expression level of S100A8 was decreased by 39% in KYSE150-TRAP1 cells. Conclusion TRAP1 is overexpressed in esophageal carcinoma and promotes the metastasis of esophageal carcinoma by regulating S100A8 ex-pression.