Anti-hemolytic, antibacterial and anti-cancer activities of methanolic extracts from leaves and stems of Polygonum odoratum

ABSTRACT

To investigate anti-hemolytic, antibacterial and anti-cancer activities of leaf and stem extracts fromPolygonum odoratum.Methods: Leaves and stems ofPolygonum odoratum were extracted using methanol and their anti-hemolytic activity was assessed using 2, 2′-Azobis (2-methylpropionamidine) dihydrochloride which is known to generate free radical damage on cell membranes of red blood cells. This damage, represented by hemolysis, was measured using spectrophotometry. Antibacterial activity was tested by using a broth microdilution method to find minimal inhibitory concentrations against eight bacterial strains. Anti-cancer activity of the extracts was evaluated against a human promyelocytic leukemic cell line (HL-60) by using MTT assay for cell viability and flow cytometry for apoptosis induction and cell cycle analysis.Results: Both leaf and stem extracts have anti-hemolytic activity. The results showed a significantly increased percentage of inhibition in a concentration-dependent manner. Interestingly, the leaf extract showed anti-hemolytic activity to a greater extent than the stem extract. Antibacterial activity of the extracts, as indicated by their minimal inhibitory concentration, using 12.5, 50, 25, 25 μg/mL, was measured againstStaphylococcus epidermidis, Enterococcus faecium,Enterococcus faecalisand Staphylococcus aureus. The leaf extracts also exhibited anti-cancer activity, demonstrated by significantly decreased cell viability of human promyelocytic cells (HL-60), with an IC50 of (350.00±1.85) μg/mL for 48 h and (38.00±0.92) μg/mL for 72 h. Additionally, HL-60 became apoptotic and accumulated in G1-phase after 48 hours of treatment.Conclusions: The extracts ofPolygonum odoratum exhibit potential anti-hemolytic activity. They also have antibacterial activity by inhibiting growth of Gram-positive bacteria. The leaf extract shows anti-cancer activity against HL-60 to a greater extent than the stem extract, causing decreased viability, increased G1-phase accumulation and apoptosis induction.