Caspases Activation in Ultraviolet B-induced Apoptosis of G361 Human Melanoma Cell Line
Annals of Dermatology
;
: 243-246, 2000.
Article
in English
| WPRIM
| ID: wpr-73470
ABSTRACT
BACKGROUND:
Ultraviolet B (UVB) irradiation can induce apoptosis of melanocytes and melanoma cells. However, mechanism of UVB-induced apoptosis of melanoma cells is not clarified yet.OBJECTIVE:
Our purpose was to study the molecular mechanism of UVB-induced apoptosis of melanoma cells.METHODS:
G361 lightly pigmented melanoma cells were analyzed for apoptotic mechanism by flow cytometry and western blotting.RESULTS:
G361 melanoma cells showed apoptotic features with gradual increment of UVB doses by MTT and flow cytometry. Western blotting disclosed activation of caspase-3 and poly (ADP-ribose) polymerase (PARP) after UVB irradiation.CONCLUSION:
In this study, we showed that UVB-induced apoptosis of melanoma cells is mediated by PARP activation which is induced by caspase cascade.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Line
/
Blotting, Western
/
Apoptosis
/
Caspases
/
Caspase 3
/
Flow Cytometry
/
Melanocytes
/
Melanoma
Limits:
Humans
Language:
English
Journal:
Annals of Dermatology
Year:
2000
Type:
Article
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