The methylation expression and clinical significance of DKK3 in gastric cancer / 中华普通外科杂志

ABSTRACT

Objective To study the correlation between status of methylation of DKK3 gene promoter and expression of its mRNA in gastric cancer.Methods Methylation of DKK3 gene promotor and its mRNA expression in 76 gastric cancer specimens and paired adjacent non-cancerous tissues were detected by methylation-specific PCR(MSP) and RT-PCR respectively.Results The expression of DKK3 mRNA in gastric cancer tissues was lower than that of adjacent non-cancerous tissues (43％ vs.60％,x2 =5.663,P =0.017).29 cases had methylation of DKK3 gene promoter for adjacent non-cancerous tissues.In gastric cancer tissues,however,44 cases had methylation of DKK3 gene promoter (38％ vs.58％,x2 =19.405,P =0.000).There were significant difference in depth of invasion (P =0.014),TNM stage (P =0.032),pathological grading (P =0.016) and lymphatic metastasis (P =0.007).There was distinct negative correlation between methylation of DKK3 gene promoter and expression of its mRNA (x2 =64.286,P =0.000).Conclusions Abnormal methylation of DKK3 gene promoter is an important mechanism of its inactivation,and might play an important role in carcinogenesis and progression of gastric cancer.