Comparative study of different biopsy methods of histopathological evaluation on donor kidney / 中华器官移植杂志

ABSTRACT

Objective To investigate the value of different biopsy methods for quality evaluation of the donated kidney organ after citizen death.Methods Six cases (6 pairs) of discard donor kidneys were collected from October 2016 to May 2017,respectively,and grouped by wedge biopsy and core needle biopsy.After being fixed and processed for routine paraffin embedding and hematoxylin-eosin (H&E) staining,the specimens were evaluated by the qualified rate of sample,the number of glomerulus,sclerotic glomerulus and small arteries.Results The comparison of two different biopsy methods showed that the number of samples obtained by wedge biopsy was 30.There were 29 samples which were qualified and the qualification rate was 96.7％.The number of samples obtained by core needle biopsy was 30,and only 21 samples were qualified and the qualification rate was 70％.In the wedge biopsy samples,the average number of glomeruli was 22.1 and 6.9 of them were sclerotic glomeruli.The ratio of sclerotic glomeruli was 31.3％.The average number of glomeruli in core needle biopsy samples was 9.5 and 2.1 of them were sclerotic glomeruli.The ratio of sclerotic glomeruli was 22.1 ％.The average number of arteries in wedge biopsy samples was 5.4,and that in core needle biopsy samples was 3.9.The results indicated that the qualification rate of wedge biopsy was significantly higher than that of core needle biopsy (P＜0.01).The number of glomeruli,sclerotic glomeruli and small arteries in wedge biopsy samples was significantly greater than than in core needle biopsy (P＜0.05).Conclusion Wedge biopsy was superior to core needle biopsy for the quality evaluation of specimens and identifying clinically significantly histopathological findings.Thus it is potential for wedge biopsy to become the main method in pre-implantation histopathological evaluation.