Expressions of glial fibrillary acidic protein, Vimentin and cytokeratin AE1/AE3 in glioma and brain metastases and their clinical significances / 肿瘤研究与临床

ABSTRACT

Objective To investigate the expressions of glial fibrillary acidic protein (GFAP),Vimentin and cytokeratin AE1/AE3 in human glioma and brain metastases and their relationship with clinicopathological features.Methods Immunohistochemistry SP method was used to detect the expression of GFAP,Vimentin and AE1/AE3 in 72 gliomas and 45 brain metastases in Shanxi Provincial Cancer Hospital from February 2013 to February 2015.The relationship between the expressions of three proteins and clinical features and pathological parameters was analyzed.Results The positive rates of GFAP and Vimentin in glioma tissues were 72.2 ％ (52/72) and 73.6 ％ (53/72),respectively,which were higher than those in brain metastatic cancer tissues [13.3 ％ (6/45) and 17.8 ％ (8/45)],and the differences were statistically significant (x2 values were 54.8 and 34.6,both P ＜ 0.001),while the positive rate of AE1/AE3 in brain metastases was 88.9 ％ (40/45),which was higher than that in human glioma tissues (6.9 ％,5/72),and the difference was statistically significant (x2 =82.2,P ＜ 0.001).The positive expression of GFAP was negatively correlated with tumor pathological grade (r =-0.57,P ＜ 0.05),while the positive expression of Vimentin was positively correlated with tumor pathological grade (r =0.62,P ＜ 0.05).The expression of GFAP in human glioma was positively correlated with Vimentin (r =0.754,P ＜ 0.001).Conclusions GFAP,Vimentin and AE1/AE3 can be used as markers for the differential diagnosis of glioma and brain metastases,especially for patients with difficult histological diagnosis.Detection of GFAP and Vimentin can help to judge the degree of malignancy and prognosis of the tumors.