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Prevalence of antiretroviral drug resistance in treatment-naive injecting drug users infected with HIV-1 in Guangzhou, 2008-2015 / 中华流行病学杂志
Chinese Journal of Epidemiology ; (12): 196-201, 2019.
Article in Chinese | WPRIM | ID: wpr-736771
ABSTRACT
Objective To understand the prevalence of drug resistance in treatment-naive injecting drug users (IDUs) infected with HIV-1 in Guangzhou.Methods HIV-1 RNA were extracted from the serum specimens of the newly confirmed HIV-1 positive IDUs living in Guangzhou,being infected through injecting drug use and receiving no antiretroviral therapy at the time of confirmation during 2008-2015.Full sequence of pol protease (PR) gene and partial sequence of reverse transcriptase (RT) gene were amplified by nested reverse transcription polymerase chain reaction (nested-PCR) and sequenced.After that,data were submitted to the HIV resistance database of Stanford University for drug resistance analysis.Results Among the 518 HIV-1 infected IDUs,H1V-1 pol gene segments were successfully obtained from the serum samples of 407 HIV-1 infected IDUs (78.57%) aged 18-64 (37.44 ± 8.14) years.Among them,males accounted for 89.68% (365/407),those of Han ethnic group accounted for 89.93% (366/407),the unmarried accounted for 55.28% (225/407),and those with education level of junior high school or below accounted for 83.78% (341/407).The distribution of subtypes was predominated by CRF07_BC (47.18%,192/407),followed by CRF01_AE (23.83%,97/407),CRF08_BC (22.85%,93/407),and other subtypes (6.14%,25/407).The overall prevalence of drug resistance was 3.44% (14/407).The prevalence of drug resistance to protease inhibitors,nucleoside reverse transcriptase inhibitors and non-nucleoside reverse transcriptase inhibitors were 1.47%(6/407),0.25% (1/407) and 1.72% (7/407) respectively.The mutation rate was 12.29% (50/407).No major drug resistance mutation was detected in protease and nucleoside reverse transcriptase regions.Higher rate of V179E mutation in the non-nucleoside reverse transcriptase region was detected in other subtypes and subtype CRF07_BC.Mutation seemed to have occurred in all 8 cases of subtype CRF55_01B in other subtypes.The highest mutation rate of E138A was detected in subtype CRF08_BC (3.23%).Two cases were resistant to all four drugs of NNRTIs.Conclusions The prevalence of drug resistance in treatment-naive HIV-1 positive IDUs remained at a relatively low level during 2008-2015,in Guangzhou.Most infections were sensitive to existing antiviral drugs.However,drug resistance surveillance in IDUs infected with HIV should be strengthened to prevent the prevalence of multi-drug resistance and cross drug resistance.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prevalence study Language: Chinese Journal: Chinese Journal of Epidemiology Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Prevalence study Language: Chinese Journal: Chinese Journal of Epidemiology Year: 2019 Type: Article