Preparation of an adenovirus expressing cytosine deaminase and observat ion on its tumor-killing effect in vitro / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 47-49, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-736799
ABSTRACT
Objective:
To prepare an adenovirus, Ad-CD, expressing bacterial cytosine deaminase(CD),and use it for tumor suicide gene therapy.Methods:
We constructed a recomb ined adenoviral vector carrying CD or LacZ. 293 packaging cell was trans fected by the newly-constructed plasmid and generated the infectious virus. The n the adenovirus was used to infect C26 cell in vitro.Results:
The growth of Ad-CD-infected cell was obviously surpressed after admini stration of 5-FC.Conclusion:
CD gene carried by adenoviral vec tor is effective for tumor cell suicide gene therapy in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2001
Type:
Article
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