Association between pancreatic cancer risk and the interaction of U2AF65 gene polymorphisms and smoking / 中华流行病学杂志

ABSTRACT

Objective To determine the association between U2 small nuclear ribonucleoprotein auxiliary factor 35/65(U2AF35 and U2AF65)and pancreatic cancer(PC). Methods A two-stage analysis case-control study was conducted. Four candidate tag single nucleotide polymorphisms (tagSNPs)were genotyped by Taqman Openarray assay in a screening population living in Central China(298 PC cases and 525 controls). Thereafter,rs310445 in U2AF65 was genotyped by TaqMan real-time polymerase chain reaction(RT-PCR)in a validation Chinese Han population from Beijing (413 cases and 557 controls). Results rs310445 in U2AF65 gene was significantly associated with PC in both screened population and combined population. Subjects with C allele had a higher risk of PC compared to those with the TT genotype,with OR of 1.31(95%CI1.07-1.60,P=0.010)for the combined population. A synergic effect of smoking and C allele of rs310445 was also observed in the combined population,with Synergic Index of 2.08(95%CI1.37-2.78) in the combined population. Conclusion Our findings suggested the interaction between smoking and U2AF65 might play a role in PC. These findings should be confirmed by further independently large-scale population studies.