Potentiating Effects of Bay K 8644 on the Relaxation Induced by Ultraviolet or Visible Light in Porcine Coronary Artery
Korean Circulation Journal
;
: 78-87, 1996.
Article
in Korean
| WPRIM
| ID: wpr-73809
ABSTRACT
BACKGROUND:
This study was aimed at defining the varying responses of porcine coronary artery(PCA) to various wavelengths of ultraviolet irradiation, and at relating them to the changes in cyclic GMP contents.METHODS:
The ring preparations of PCA with intact or removed endothelium were irradiated with the ultraviolet or visible light of wavelengths(240-520mm) from xenon lamp of a spectrofluorometer, and the changes in vascular tension were recorder on polygraph. For cyclic GMP assay, rat thoracic aorta was frozen after irradiation and homogenated. The supernatant was extracted with water-saturated ether and the cyclic GMP contents were measured with radioimmunoassay.RESULTS:
Ultraviolet irradiation relaxed the preparations(UVR-relaxation) in resting state and those precontracted by prostaglandin F2alpha, the maximal relaxation occurring at 410nm, and the magnitude depending on the duration of irradiation. The UVR-relaxation was not affected by removing the endothelium, while it was markedly potentiated by pretreatment with Bay K 8644. The Bay K 8644-induced potentiation of UVR-relaxation was abolished by hemoglobin and slightly reduced by wrapping the rings with aluminum foil. Cyclic GMP contents in the increase was markedly potentiated by pretreatment with Bay K 8644.CONCLUSION:
The observations suggest that UVR-relaxation in procine coronary artery is caused by activating the nitric oxide-cyclic GMP system, which is most sensitively activated by UVR of 410nm and that its potentiation induced by Bay K 8644 may be related nitrous substance released from the agent upon UVR.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta, Thoracic
/
Relaxation
/
Ultraviolet Rays
/
Vasodilation
/
Xenon
/
Passive Cutaneous Anaphylaxis
/
Radioimmunoassay
/
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
/
Dinoprost
/
Cyclic GMP
Limits:
Animals
Language:
Korean
Journal:
Korean Circulation Journal
Year:
1996
Type:
Article
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