Effects of Valproic Acid on the Survival of Human Tennon's Capsule Fibroblasts
Journal of the Korean Ophthalmological Society
;
: 1056-1061, 2018.
Article
in Korean
| WPRIM
| ID: wpr-738491
ABSTRACT
PURPOSE:
To investigate the effects of valproic acid on the survival of cultured human Tenon's capsule fibroblasts (HTFBs).METHODS:
Primary cultured HTFBs were exposed to 0, 0.25, 0.5, and 1.0 mM valproic acid with or without 0, 1.0, 2.5 µg/mL mitomycin C, and incubated for 5 days. Cell survival was assessed using an MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and the degree of apoptosis was assessed by flow cytometry using annexin-V/propidium iodide double staining.RESULTS:
Valproic acid decreased the survival of HTFBs in a dose-dependent manner, and survival was further decreased by adding mitomycin C to valproic acid. Both valproic acid and mitomycin C induced apoptosis of HTFBs. Valproic acid induced less apoptosis than mitomycin C.CONCLUSIONS:
Valproic acid decreased the cellular survival of HTFBs and induced apoptosis. The antiproliferative effects of valproic acid were further enhanced by the addition of mitomycin C.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Survival
/
Valproic Acid
/
Mitomycin
/
Apoptosis
/
Tenon Capsule
/
Fibroblasts
/
Flow Cytometry
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2018
Type:
Article
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