Effects of High Glucose and Dexamethasone on the Permeability in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
; : 252-260, 2018.
Article
in Ko
| WPRIM
| ID: wpr-738520
Responsible library:
WPRO
ABSTRACT
PURPOSE: To investigate the effects of high glucose (HG) and dexamethasone (DEX) on the survival and permeability of trabecular meshwork cells (HTMC), and associated changes in tight junctions. METHODS: Primary cultured HTMC were exposed to 5 mM low glucose (LG) or 25 mM HG with or without 1.0 µM DEX for 3 days. The permeability of the HTMC monolayer was assessed using carboxyfluorescein or transendothelial electrical resistance (TEER). Gene and protein expressions of claudin-5 and occludin were assessed with reverse transcription polymerase chain reaction (RT-PCR) and Western blot, respectively. RESULTS: HG was significantly associated with greater HTMC monolayer permeability compared to LG by both the carboxyfluorescein permeability test and TEER (p = 0.022, 0.028). HG also decreased claudin-5 and occludin mRNA expression, respectively (7.5%, 12.9%). DEX abolished HG-induced increased permeability, and increased the protein expression of claudin-5 and occludin, respectively (p = 0.015, 0.012). CONCLUSIONS: In HTMCs, DEX reversed HG-induced permeability increase. DEX increased tight junction molecules claudin-5 and occludin. Thus, DEX-induced changes in junctional proteins could be another mechanism of increased resistance through the trabecular meshwork and may result in steroid-induced glaucoma.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Permeability
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Trabecular Meshwork
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RNA, Messenger
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Dexamethasone
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Glaucoma
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Blotting, Western
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Polymerase Chain Reaction
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Electric Impedance
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Tight Junctions
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Reverse Transcription
Language:
Ko
Journal:
Journal of the Korean Ophthalmological Society
Year:
2018
Type:
Article