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Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine / 전남의대학술지
Chonnam Medical Journal ; : 63-71, 2018.
Article in English | WPRIM | ID: wpr-739310
ABSTRACT
Purinergic receptors play an important role in regulating gastrointestinal (GI) motility. Interstitial cells of Cajal (ICCs) are pacemaker cells that regulate GI smooth muscle activity. We studied the functional roles of external adenosine 5′-triphosphate (ATP) on pacemaker activity in cultured ICCs from mouse small intestines by using the whole-cell patch clamp technique and intracellular Ca²⁺ ([Ca²⁺]ᵢ) imaging. External ATP dose-dependently depolarized the resting membrane and produced tonic inward pacemaker currents, and these effects were antagonized by suramin, a purinergic P2 receptor antagonist. ATP-induced effects on pacemaker currents were suppressed by an external Na⁺-free solution and inhibited by the nonselective cation channel blockers, flufenamic acid and niflumic acid. The removal of external Ca²⁺ or treatment with thapsigargin (inhibitor of Ca²⁺ uptake into endoplasmic reticulum) inhibited the ATP-induced effects on pacemaker currents. Spontaneous [Ca²⁺]ᵢ oscillations were enhanced by external ATP. These results suggest that external ATP modulates pacemaker activity by activating nonselective cation channels via external Ca²⁺ influx and [Ca²⁺]ᵢ release from the endoplasmic reticulum. Thus, it seems that activating the purinergic P2 receptor may modulate GI motility by acting on ICCs in the small intestine.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pacemaker, Artificial / Suramin / Adenosine / Adenosine Triphosphate / Flufenamic Acid / Niflumic Acid / Receptors, Purinergic / Receptors, Purinergic P2 / Thapsigargin / Endoplasmic Reticulum Limits: Animals Language: English Journal: Chonnam Medical Journal Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pacemaker, Artificial / Suramin / Adenosine / Adenosine Triphosphate / Flufenamic Acid / Niflumic Acid / Receptors, Purinergic / Receptors, Purinergic P2 / Thapsigargin / Endoplasmic Reticulum Limits: Animals Language: English Journal: Chonnam Medical Journal Year: 2018 Type: Article