Evaluation of periopathogenic bacteria of peri-implantitis using real-time PCR in Koreans: pilot study
Journal of Dental Rehabilitation and Applied Science
;
: 186-195, 2018.
Article
in Korean
| WPRIM
| ID: wpr-739881
ABSTRACT
PURPOSE:
We performed quantitative and qualitative analysis of typical periodontal bacteria using real time PCR method to investigate the microbiological difference according to the severity of peri-implant disease in Koreans. MATERIALS ANDMETHODS:
Total of 60 implants were divided into three groups (healthy group, peri-implant mucositis group, peri-implantitis group) through periapical radiographs and clinical indices. The evaluated clinical parameters were pocket depth, plaque index, suppuration and bleeding on probing. Using a sterilized curette instrument, microbial samples were collected from the subgingival plaque and real-time PCR was performed on five periodontal bacteria. The relative expression levels of microorganisms were compared by comparative delta-CT method.RESULTS:
The relative expression levels of E. corrodens and T. denticola were significantly higher in the peri-implantitis group (P < 0.017). On the other hand, the relative expression level of F. nucleatum and P. gingivalis was relatively high in the healthy implant group regardless of the severity of disease. P. intermedia was significantly lower in the healthy implant group (P < 0.017).CONCLUSION:
Periodontal bacteria were detected in Koreans with peri-implant diseases, but there was no microbiological distribution similar to periodontitis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontitis
/
Suppuration
/
Bacteria
/
Pilot Projects
/
Asian People
/
Mucositis
/
Peri-Implantitis
/
Real-Time Polymerase Chain Reaction
/
Microbiota
/
Hand
Type of study:
Qualitative research
Limits:
Humans
Language:
Korean
Journal:
Journal of Dental Rehabilitation and Applied Science
Year:
2018
Type:
Article
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