Large-scale Isolation, Expansion and Characterization of Human Amniotic Epithelial Cells
International Journal of Stem Cells
;
: 87-95, 2018.
Article
in English
| WPRIM
| ID: wpr-739918
ABSTRACT
BACKGROUND AND OBJECTIVES:
The human Amniotic epithelial cells (AME) derived from amniotic membrane of placenta have been considered as the potential fetal stem cell source with minimal or no ethical concerns and are important therapeutic tool for anti-fibrotic and regenerative therapies. METHODS ANDRESULTS:
Here, we evaluated the isolation, media screening, scale-up and characterization of AME cells. The isolation, expansion of AMEs were performed by sequential passaging and growth kinetics studies. The AMEs were characterized using immunocytochemistry, immunophenotyping, In-vitro differentiation, and anti-fibrotic assays. The growth kinetics study revealed that the AME cultured in Ultraculture (UC) and DMEM knockout (DMEM-KO) have prominently higher growth rate compared to others. Overall, the AMEs cultured from 5 different media retained basic morphological characteristics and the functional characteristics.CONCLUSIONS:
Our result suggests that the AMEs can be successfully cultured in UC based complete media without losing its epithelial cell characteristics even after passaging for passage 2 (P2). However, a careful and methodical pre-clinical and clinical translation studies need to be conducted to show its safety and efficacy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Immunohistochemistry
/
Kinetics
/
Cryopreservation
/
Mass Screening
/
Immunophenotyping
/
Tissue Engineering
/
Epithelial Cells
/
Fetal Stem Cells
/
Cell- and Tissue-Based Therapy
Type of study:
Prognostic study
/
Screening study
Limits:
Humans
Language:
English
Journal:
International Journal of Stem Cells
Year:
2018
Type:
Article
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