Hypoxic Preconditioned Mesenchymal Stromal Cell Therapy in a Rat Model of Renal Ischemia-reperfusion Injury: Development of Optimal Protocol to Potentiate Therapeutic Efficacy
International Journal of Stem Cells
;
: 157-167, 2018.
Article
in English
| WPRIM
| ID: wpr-739931
ABSTRACT
Although previous and ongoing clinical studies have used stromal cells during renal ischemia-reperfusion injury (IRI), there is little consensus regarding the optimal protocol. We aimed to optimize the protocol for hypoxic preconditioned human bone marrow-derived mesenchymal stromal cell (HP-hBMSC) therapy in a rat model of renal IRI. We determined the optimal injection route (renal arterial, renal parenchymal, and tail venous injection), dose (low-dose 1×10⁶, moderate-dose 2×10⁶, and high-dose 4×10⁶), and injection period (pre-, concurrent-, and post-IRI). During optimal injection route study, renal arterial injections significantly reduced the decreasing glomerular filtration rate (GFR), as compared to GFRs for the IRI control group, 2 and 4 days after IRI. Therapeutic effects and histological recoveries were the greatest in the group receiving renal arterial injections. During the dose finding study, high-dose injections significantly reduced the decreasing GFR, as compared to GFRs for the IRI control group, 3 days after IRI. Therapeutic effects and histological recoveries were the greatest in the high-dose injection group. While determining the optimal injection timing study, concurrent-IRI injection reduced elevated serum creatinine levels, as compared to those of the IRI control group, 1 day after IRI. Pre-IRI injection significantly reduced the decreasing GFR, as compared with GFRs for the IRI control group, 1 day after IRI. Therapeutic effects and histological recoveries were the greatest in the concurrent-IRI group. In conclusion, the concurrent-IRI administration of a high dose of HP-hBMSC via the renal artery leads to an optimal recovery of renal function after renal IRI.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Renal Artery
/
Tail
/
Reperfusion Injury
/
Stromal Cells
/
Creatinine
/
Models, Animal
/
Consensus
/
Therapeutic Uses
/
Acute Kidney Injury
/
Mesenchymal Stem Cells
Type of study:
Practice guideline
/
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
International Journal of Stem Cells
Year:
2018
Type:
Article
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