Identification of Epithelial-Mesenchymal Transition-related Target Genes Induced by the Mutation of Smad3 Linker Phosphorylation
Journal of Cancer Prevention
;
: 1-9, 2018.
Article
in English
| WPRIM
| ID: wpr-740095
ABSTRACT
BACKGROUND:
Smad3 linker phosphorylation plays essential roles in tumor progression and metastasis. We have previously reported that the mutation of Smad3 linker phosphorylation sites (Smad3-Erk/Pro-directed kinase site mutant constructs [EPSM]) markedly reduced the tumor progression while increasing the lung metastasis in breast cancer.METHODS:
We performed high-throughput RNA-Sequencing of the human prostate cancer cell lines infected with adenoviral Smad3-EPSM to identify the genes regulated by Smad3-EPSM.RESULTS:
In this study, we identified genes which are differentially regulated in the presence of Smad3-EPSM. We first confirmed that Smad3-EPSM strongly enhanced a capability of cell motility and invasiveness as well as the expression of epithelial-mesenchymal transition marker genes, CDH2, SNAI1, and ZEB1 in response to TGF-β1 in human pancreatic and prostate cancer cell lines. We identified GADD45B, CTGF, and JUNB genes in the expression profiles associated with cell motility and invasiveness induced by the Smad3-EPSM.CONCLUSIONS:
These results suggested that inhibition of Smad3 linker phosphorylation may enhance cell motility and invasiveness by inducing expression of GADD45B, CTGF, and JUNB genes in various cancers.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreatic Neoplasms
/
Phosphorylation
/
Phosphotransferases
/
Prostatic Neoplasms
/
Breast Neoplasms
/
Cell Line
/
Cell Movement
/
Sequence Analysis, RNA
/
Epithelial-Mesenchymal Transition
/
Lung
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Journal of Cancer Prevention
Year:
2018
Type:
Article
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