Effects of Ethyl Pyruvate on Allodynia, TNF-alpha Expression, and Apoptosis in the Dorsal Root Ganglion after Spinal Nerve Ligation Injury
The Korean Journal of Pain
;
: 213-220, 2012.
Article
in English
| WPRIM
| ID: wpr-74032
ABSTRACT
BACKGROUND:
It has been demonstrated that the expression of tumor necrosis factor-alpha (TNF-alpha) and apoptotic cell death in the dorsal root ganglion (DRG) following spinal nerve constriction injury play a role in the initiation and continuation of hyperalgesia and allodynia. The present study was designed to investigate the effects of ethyl pyruvate (EP) on mechanical and cold allodynia, TNF-alpha expression, and apoptosis in DRG after spinal nerve ligation injury.METHODS:
Rats were divided into 3 groups control, pre-EP, and post-EP. EP (50 mg/kg) was intraperitoneally injected 30 minutes before (pre-EP) or after (post-EP) surgery. Behavioral tests to determine mechanical and cold allodynia were conducted before surgery and 4 and 7 days after surgery. Seven days after surgery, TNF-alpha protein levels in DRG were evaluated by enzyme-linked immunosorbent assay, and DRG apoptosis was determined by immunohistochemical detection of activated caspase-3.RESULTS:
Treatment with EP significantly reduced mechanical and cold allodynia following spinal nerve ligation injury. TNF-alpha protein levels in the pre-EP (4.7 +/- 1.2 pg/200 microg; P < 0.001) and post-EP (6.4 +/- 1.8 pg/200 microg; P < 0.001) groups were 2-3 times lower than the control group (14.4 +/- 1.2 pg/200 microg). The percentages of neurons and satellite cells that co-localized with caspase-3 were also significantly lower in the pre-EP and post-EP groups than the control group.CONCLUSIONS:
These results demonstrate that EP has a strong anti-allodynic effect that acts through the inhibition of TNF-alpha expression and apoptosis in DRG after spinal nerve ligation injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pyruvates
/
Spinal Nerve Roots
/
Spinal Nerves
/
Enzyme-Linked Immunosorbent Assay
/
Tumor Necrosis Factor-alpha
/
Cell Death
/
Diagnosis-Related Groups
/
Apoptosis
/
Cold Temperature
/
Constriction
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Pain
Year:
2012
Type:
Article
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