Anti-melanogenic effects of Hordeum vulgare L. barely sprout extract in murine B16F10 melanoma cells / 한국영양학회지
Journal of Nutrition and Health
;
: 168-175, 2019.
Article
in English
| WPRIM
| ID: wpr-740563
ABSTRACT
PURPOSE:
Barely sprout is a well-known oriental herbal medicine with a wide range of health benefits. Recent studies have provided scientific evidence of its therapeutic effects with expanded application. This study investigated anti-melanogenic effect of barley sprout water extract (BSE) in murine melanocyte B16F10.METHODS:
Various concentrations (0, 50, 125, and 250 µg/mL) of BSE and arbutin (150 ppm) were applied to B16F10 stimulated with or without alpha-melanocyte stimulating hormone (100 nM) for 72 hours. The whitening potency of BSE was determined altered cellular melanin contents. Activity and expression of tyrosinase and microphthalmia-associated transcription factor (MITF) were also assayed.RESULTS:
Experimental results revealed that treatment with BSE reduced cellular melanin production by approximately 40% compared to the control. Molecular findings supported that suppressed activity and expression of tyrosinase and MITF proteins by BSE were associated with declined cellular melanogenesis. Furthermore, anti-melanogenic effect of BSE (250 µg/mL) was similar to that of arbutin, a commonly used whitening agent. Lastly, polyphenols including p-coumaric, ferulic, and vanillic acids were identified in BSE using HPLC analyses. They might be potential active ingredients showing such melanogenesis-reducing effect.CONCLUSION:
BSE was evident to possess favorable anti-melanogenic potency in an in vitro model. As a natural food sourced material, BSE could be an effective depigmentation agent with potential application in pharmaceutical and cosmetic industries.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arbutin
/
Hordeum
/
Vanillic Acid
/
In Vitro Techniques
/
Water
/
Chromatography, High Pressure Liquid
/
Monophenol Monooxygenase
/
Herbal Medicine
/
Therapeutic Uses
/
Microphthalmia-Associated Transcription Factor
Type of study:
Prognostic study
Language:
English
Journal:
Journal of Nutrition and Health
Year:
2019
Type:
Article
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