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Antioxidant and growth inhibitory activities of Mesembryanthemum crystallinum L. in HCT116 human colon cancer cells / 한국영양학회지
Journal of Nutrition and Health ; : 157-167, 2019.
Article in Korean | WPRIM | ID: wpr-740564
ABSTRACT

PURPOSE:

This study examined the antioxidant and cancer cell growth inhibitory activities of an ethanol extract and different solvent fractions of Mesembryanthemum crystallinum L. (ice plant).

METHODS:

The ice plant was freeze-dried, extracted with 99.9% ethanol, and then fractionated with hexane, ethyl acetate, butanol, and water. The total polyphenol content (TPC), total carotenoid content (TCC), 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity (RSA), and ferric reducing antioxidant power (FRAP) were measured. Assays using 2′,7′-dichlorofluorescin-diacetate and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide were performed to measure the intracellular reactive oxygen species (ROS) and cell growth, respectively. Annexin V/propidium iodide staining and cell cycle analysis were performed for the detection of apoptosis and cell cycle arrest.

RESULTS:

TPC, TCC, RSA, and FRAP of the ethanol extract (EE) were 3.7 mg gallic acid equivalent/g, 13.2 µg/g, 21.0% (at a concentration of 5 mg/mL), and 21.0% (at a concentration of 5 mg/mL), respectively. Among the different solvent fractions, the butanol fraction (BF) showed the highest TPC (5.4 mg gallic acid equivalent/g), TCC (86.6 µg/g), RSA (34.9% at 5 mg/mL), and FRAP (80.8% at 5 mg/mL). Treatment of HCT116 human colon cancer cells with EE and BF at concentrations of 250 and 500 µg/mL reduced the levels of intracellular ROS. Concomitantly, EE and BF resulted in the dose-dependent inhibition of cell growth (at the concentrations of 125, 250, and 500 µg/mL for 24 ~ 48 h) and the induction of apoptosis (at the concentrations of 250 and 500 µg/mL for 48 h) in HCT116 cells. An increased G2/M cell population was also found in the BF-treated cells.

CONCLUSION:

These results suggest that ice plant possesses antioxidant and growth inhibitory activities in colon cancer cells.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Water / Cell Cycle / Reactive Oxygen Species / Apoptosis / Colon / Colonic Neoplasms / Mesembryanthemum / HCT116 Cells / Ethanol / Cell Cycle Checkpoints Limits: Humans Language: Korean Journal: Journal of Nutrition and Health Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Water / Cell Cycle / Reactive Oxygen Species / Apoptosis / Colon / Colonic Neoplasms / Mesembryanthemum / HCT116 Cells / Ethanol / Cell Cycle Checkpoints Limits: Humans Language: Korean Journal: Journal of Nutrition and Health Year: 2019 Type: Article