Molecular identification of coagulase-negative staphylococci by rpoB sequence typing / 대한수의학회지
Korean Journal of Veterinary Research
;
: 51-55, 2018.
Article
in Korean
| WPRIM
| ID: wpr-741487
ABSTRACT
Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacterial Infections
/
Epidemiology
/
Genes, rRNA
/
Diagnosis
/
Mastitis, Bovine
/
Methods
Type of study:
Diagnostic study
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Veterinary Research
Year:
2018
Type:
Article
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