Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus / 대한수의학회지
Korean Journal of Veterinary Research
;
: 27-31, 2018.
Article
in English
| WPRIM
| ID: wpr-741491
ABSTRACT
Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 10⁴/mL and 9.85 × 10³/mL for samples with a 2-fold serial dilution from 1.25 × 10⁶ TCID₅₀ (TCID₅₀, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Kits, Diagnostic
/
RNA Viruses
/
Immunoassay
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Parvovirus, Canine
/
Coronavirus, Canine
/
Coronavirus
/
Diagnostic Tests, Routine
/
Distemper
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Korean Journal of Veterinary Research
Year:
2018
Type:
Article
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