Comparison of hemostatic efficacy and cytotoxicity of three ferric subsulfate- and chitosan-based styptics in different formulations using a rat tail bleeding model / 대한수의학회지
Korean Journal of Veterinary Research
;
: 119-124, 2018.
Article
in English
| WPRIM
| ID: wpr-741514
ABSTRACT
This study was conducted to compare the hemostatic efficacy of three ferric subsulfate- and chitosan-based styptics as a powder and a gel containing ferric subsulfate and chitosan (FSC-PO and FSC-G, respectively) and a soaked pad containing ferric subsulfate and lidocaine (FSL-SP) using a rat tail bleeding model. The cytotoxicity of the styptics against L-929 mouse fibroblasts was also evaluated using a cell counting kit-8 assay. Four groups of 10 rats each were assigned to the three different styptics and a non-treated control groups. Rat tail tips were transected, after which styptics were applied with pressure. The wounds were observed for hemostasis for 3 min, then irrigated with saline to check for recurrent hemorrhage. L-929 mouse fibroblasts were exposed to extracts of the styptics (100 mg/mL) and their dilutions (110, 1100, and 11,000). FSC-PO and FSC-G more effectively controlled initial hemorrhage than FSL-SP (p = 0.033). Additionally, FSC-PO and FSC-G more effectively maintained hemostasis than the control group (p = 0.02 and p < 0.01, respectively). However, all styptics showed enhanced cytotoxicity against L-929 cells in a dose-dependent manner. Therefore, although FSC-PO and FSC-G would be recommended to control hemorrhage, the benefits of styptics must be balanced against the clinical significance of their cytotoxicity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tail
/
Wounds and Injuries
/
Hemostatics
/
Cell Count
/
Cytotoxicity, Immunologic
/
Chitosan
/
Fibroblasts
/
Hemorrhage
/
Hemostasis
/
Lidocaine
Limits:
Animals
Language:
English
Journal:
Korean Journal of Veterinary Research
Year:
2018
Type:
Article
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