Isolation and identification of canine adenovirus type 2 from a naturally infected dog in Korea / 대한수의학회지
Korean Journal of Veterinary Research
;
: 177-182, 2018.
Article
in English
| WPRIM
| ID: wpr-741522
ABSTRACT
Canine adenovirus type 2 (CAV-2) infection results in significant respiratory illness in dogs. Isolating and culturing CAV-2 allows for investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated a virus from a naturally infected dog in Gyeonggi-do, Korea. The virus was propagated in Madin-Darby canine kidney (MDCK) and Vero cells and showed a specific cytopathic morphology that appeared similar to a bunch of grapes. The virus was first confirmed as CAV-2 based on these cytopathic effects, an immunofluorescence assay, hemagglutination assay, and electron microscopy. The viral titer of the isolate designated APQA1601 reached 10(6.5) 50% tissue culture infections dose per mL in MDCK cells and exhibited no hemagglutination units with erythrocytes from guinea pig. The virus was also confirmed by polymerase chain reaction and next-generation sequencing. The APQA1601 strain had the highest similarity (~99.9%) with the Toronto A26/61 strain, which was isolated in Canada in 1976 when the nucleotide sequences of the full genome of the APQA1601 strain were compared with those of other CAV strains. Isolating CAV-2 will help elucidate the biological properties of CAV-2 circulating in Korean dogs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vero Cells
/
Canada
/
Vaccines
/
Base Sequence
/
Microscopy, Electron
/
Polymerase Chain Reaction
/
Fluorescent Antibody Technique
/
Genome
/
Adenoviruses, Canine
/
Vitis
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
North America
/
Asia
Language:
English
Journal:
Korean Journal of Veterinary Research
Year:
2018
Type:
Article
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