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Development of an experimental model for radiation-induced inhibition of cranial bone regeneration / 대한악안면성형재건외과학회지
Maxillofacial Plastic and Reconstructive Surgery ; : 34-2018.
Article in English | WPRIM | ID: wpr-741547
ABSTRACT

BACKGROUND:

Radiation therapy is widely employed in the treatment of head and neck cancer. Adverse effects of therapeutic irradiation include delayed bone healing after dental extraction or impaired bone regeneration at the irradiated bony defect. Development of a reliable experimental model may be beneficial to study tissue regeneration in the irradiated field. The current study aimed to develop a relevant animal model of post-radiation cranial bone defect.

METHODS:

A lead shielding block was designed for selective external irradiation of the mouse calvaria. Critical-size calvarial defect was created 2 weeks after the irradiation. The defect was filled with a collagen scaffold, with or without incorporation of bone morphogenetic protein 2 (BMP-2) (1 μg/ml). The non-irradiated mice treated with or without BMP-2-included scaffold served as control. Four weeks after the surgery, the specimens were harvested and the degree of bone formation was evaluated by histological and radiographical examinations.

RESULTS:

BMP-2-treated scaffold yielded significant bone regeneration in the mice calvarial defects. However, a single fraction of external irradiation was observed to eliminate the bone regeneration capacity of the BMP-2-incorporated scaffold without influencing the survival of the animals.

CONCLUSION:

The current study established an efficient model for post-radiation cranial bone regeneration and can be applied for evaluating the robust bone formation system using various chemokines or agents in unfavorable, demanding radiation-related bone defect models.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Regeneration / Skull / Bone Regeneration / Collagen / Chemokines / Models, Animal / Bone Morphogenetic Protein 2 / Head and Neck Neoplasms / Models, Theoretical Limits: Animals Language: English Journal: Maxillofacial Plastic and Reconstructive Surgery Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Regeneration / Skull / Bone Regeneration / Collagen / Chemokines / Models, Animal / Bone Morphogenetic Protein 2 / Head and Neck Neoplasms / Models, Theoretical Limits: Animals Language: English Journal: Maxillofacial Plastic and Reconstructive Surgery Year: 2018 Type: Article