Efficient Isolation of Dihydrophaseic acid 3′-O-β-D-Glucopyranoside from Nelumbo nucifera Seeds Using High-performance Countercurrent Chromatography and Reverse-phased High-performance Liquid Chromatography
Natural Product Sciences
;
: 288-292, 2018.
Article
in English
| WPRIM
| ID: wpr-741628
ABSTRACT
High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3′-O-β-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6410, v/v/v, normal-phase mode, flow rate 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Chromatography
/
Chromatography, Liquid
/
Countercurrent Distribution
/
Nelumbo
/
Methods
Language:
English
Journal:
Natural Product Sciences
Year:
2018
Type:
Article
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