Identification and HPLC Quantification of a Phytoecdysone and Three Phenolic Glycosides in Lamium takesimense Nakai
Natural Product Sciences
; : 241-246, 2018.
Article
in En
| WPRIM
| ID: wpr-741635
Responsible library:
WPRO
ABSTRACT
The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 µm, 4.6 mm × 250 mm) with a gradient elution of H₂O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order: lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g).
Key words
Full text:
1
Index:
WPRIM
Main subject:
Plants
/
Rutin
/
Chromatography, High Pressure Liquid
/
Acetic Acid
/
Lamiaceae
/
Phenol
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Ecdysone
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Ecdysterone
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Glycosides
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Methods
Type of study:
Diagnostic_studies
Language:
En
Journal:
Natural Product Sciences
Year:
2018
Type:
Article