Study on the Marker Steroids of New Zealand Deer (Cervus elaphus var. scoticus) Velvet Antler by UPLC-MS/MS and HPLC-PDA Methods
Natural Product Sciences
;
: 49-58, 2019.
Article
in English
| WPRIM
| ID: wpr-741644
ABSTRACT
Eleven steroid hormones (SHs androstene-3,17-dione, estrone, β-estradiol, α-estradiol, testosterone, dehydroepiandrosterone, 17á-hydroxyprogesterone, medroxyprogesterone, megestrol acetate, progesterone, and androsterone) were detected from New Zealand deer (Cervus elaphus var. scoticus) velvet antler (NZA, 鹿茸). A method for the quantification of eleven SHs was established by using ultraperformance liquid chromatography (UPLC)-MS/MS. The linearities (R² > 0.991), limits of quantification (LOQ values, 0.3 ng/mL to 23.1 ng/mL), intraday and interday precisions (relative standard deviation RSD 0.999), LOQ values (30 ng/mL to 350 ng/mL), intraday and interday precisions (RSD < 1.93%), and recovery rates (97.2% to 103.5%) for the three 7-O-CSs were determined. These quantitative methods are accurate, precise, and reproducible. As a result, it is suggested that the five steroid compounds of androstene-3,17-dione, androsterone, 7-ketocholesterol, 7α-hydroxycholesterol, and 7β-hydroxycholesterol could be marker steroids of NZA. These methods can be applied to quantify or standardize the marker steroids present in NZA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antlers
/
Progesterone
/
Steroids
/
Testosterone
/
Deer
/
Chromatography, Liquid
/
Dehydroepiandrosterone
/
Megestrol Acetate
/
Estrone
/
Androsterone
Limits:
Animals
Country/Region as subject:
Oceania
Language:
English
Journal:
Natural Product Sciences
Year:
2019
Type:
Article
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