Functional and Phenotypic Characterization of Cord Blood-Derived Dendritic Cells / 대한소아혈액종양학회지
Korean Journal of Pediatric Hematology-Oncology
;
: 217-227, 2004.
Article
in Korean
| WPRIM
| ID: wpr-74198
ABSTRACT
PURPOSE:
Hematopoietic stem cells from umbilical cord blood are one of the useful resources for stem cell transplantation in the various adult and childhood diseases. Immunologic complications of transplantation, e.g., graft-vs-host disease, occur much less with transplantation of cord blood stem cells. Cord blood-derived dendritic cells (CB-DCs) are known to be different from adult peripheral blood-derived dendritic cells (PB-DCs) in immunologic characteristics. These phenomena might be related to the characteristics of hematopoietic cells in cord blood. Therefore, we analysed characteristics of dendritic cells, which are well-known immune-provoking cells, derived from cord blood precursors.METHODS:
Dendritic cells were differentiated from plastic-adherent cord blood monocytes in the presence of GM-CSF and IL-4. Immunophenotype was analysed by flow cytometry and expression of IDO (indoleamine 2, 3-dioxygenase), an enzyme expressed in immune-regulating or tolerogenic DCs, IL-12, IL-10 and IL-6 was measured by RT-PCR along in vitro differentiation. Changes in expression of cytokines and IDO after antibody engagement were also analysed.RESULTS:
CB-DCs were very similar to PB-DCs in immunophenotype and expression of cytokines. But CB-DCs expressed IDO transcripts much earlier than PB-DCs during differentiation from precursors. Engagement of CB-DCs with DU-1 mAb induced upregulation of IDO and downregulation of IL-6.CONCLUSION:
Although immunophenotype and cytokine expression pattern of CB-DCs were quite similar to those of PB-DCs, CB-DCs expressed IDO earlier than PB-DCs. This might be related to the phenomena that CB-DCs are less immunogenic or, sometimes, tolerance-inducing.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
Dendritic Cells
/
Hematopoietic Stem Cells
/
Monocytes
/
Down-Regulation
/
Up-Regulation
/
Cytokines
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Interleukin-4
/
Interleukin-6
Limits:
Adult
/
Humans
Language:
Korean
Journal:
Korean Journal of Pediatric Hematology-Oncology
Year:
2004
Type:
Article
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