Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis
The Korean Journal of Parasitology
;
: 33-38, 2019.
Article
in English
| WPRIM
| ID: wpr-742308
ABSTRACT
Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTRPV), as a promising immunogen in BALB/c mice. First, TvTRPV was cloned and expressed as a recombinant protein in Escherichia coli BL21 cells and purified by nickel affinity. Next, BALB/c mice were immunized and the antibody levels in mice serum and cytokines from the supernatant of macrophages and from co-culture systems were evaluated. Recombinant TvTRPV triggered high levels of specific total IgG in sera from the immunized mice. Also, a statistically significant increase of cytokines IL-1β, IL-6, and TNF-α after stimulation with the corresponding antigens in vitro, was identified. Moreover, co-cultures using CD4⁺ T cells from immunized mice were able to identify higher levels of IL-10 and IFN-γ. These results were useful to validate the immunogenicity of TvTRPV in BALB/c mice, where IL-10-IFN-γ-secreting cells could play a role in infection control, supporting the potential of TvTRPV as a promising target for vaccine against T. vaginalis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parasites
/
Trichomonas
/
Trichomonas vaginalis
/
In Vitro Techniques
/
Immunoglobulin G
/
T-Lymphocytes
/
Sexually Transmitted Diseases
/
Calcium
/
Cytokines
/
Interleukin-6
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2019
Type:
Article
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